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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which ends up in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two further splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind especially towards the concentrate on antibody and block antibody binding. These peptide commonly consists of the epitope recognized from the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to ensure the very best effects are...
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This guidebook outlines further more probable optimization of mobile and immunoassay parameters to ensure the very best success are...
Antibodies bound to the blocking peptide now not bind towards the epitope over the concentrate on protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is specific.
Focus on Data Cyclin E belongs to your highly conserved cyclin family members, whose associates are characterized by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary which is degraded as cells progress as a result of S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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